Table des matières

Fixatives

Fixative for electron microscopy

Routine Fixatives

Components: https://www.emsdiasum.com/

  1. Make 0.2M cacodylate stock solution, pH 7.2-7.4
  2. Add :
    • 20ml Cacodylate 0.2M (final 0.1M)
    • 10 ml Formaldehyde 16% (4% final)
    • 4 ml Glutaraldehyde 25% (2.5% final)
    • 6 ml H20 (to complete to 40 ml)