Colorations pour l'histologie

Bleu Alcian

• Acid mucins (sulfomucins and sialomucins) blue
• Proteoglycans and hyaluronic acid blue
• Nuclei red

Brown-Brenn/Gram

• Gram-positive organisms, fibrin, some fungi, Paneth cell granules, kerato-hyalin, and keratin blue
• Gram-negative organisms red
• Nuclei red
• Other tissue elements yellow

Laidlaw

• Reticular fibers black
• Nuclei black

PAS/PASD

• Glycogen (It’s presence can be demonstrated using a a-amylase digested second slide) , neutral/sialomucins magenta
• Various glycoproteins magenta
• Nuclei blue

Thioflavine T

• Using a UV light source (mercury vapor lamp), UG1 Exciter filter, BG38 red suppression filter, and K430 barrier filter: amyloid, elastic tissue, etc. exhibit silver-blue fluorescence.
• Using blue light fluorescence quartz-iodine or mercury vapor lamp with BG12 exciter filter and K530 barrier filter: amyloid, elastic tissue, etc. exhibit yellow fluorescence.

Trichrome de Masson

• Nuclei blue/black
• Cytoplasm, muscle, and erythrocytes red
• Collagen blue

Whartin-Starry

• Spirochetes black
• Background golden/yellow

Ziehl

• Mycobacteria, hair shafts, Russell bodies, Splendore-Hoeppli immunoglobulins around actinomyces and some fungal organisms red
• Background pale blue

Méthénamine de Gomori

• Fungi, Pneumocystis, melanin black
• Hyphae and yeast-form cells sharply delineated in black of fungi
• Mucins and glycogen taupe to dark gray/ brown
• Background pale green

Rouge Congo

• Amyloid red Elastic and collagen fibers, eosinophil granules pink
• Nuclei blue

Bleu de Prusse/ Bleu de Turnbull (Fe3+/Fe2+)

• Ferric/ferrous iron blue
• Nuclei red

Bleu de Toluidine

• Éléments métachromatiques tissulaires : Rouges à roses
• Autres éléments tissulaires : Bleus à bleu-violets

HE

• Nuclei blue/black
• Cytoplasm varying shades of pink
• Muscle fibers deep pink/red
• Red blood cells orange/red
• Fibrin deep pink

HPS

• Noyaux : Bleus
• Cytoplasme, érythrocytes, fibres élastiques et musculaires : Rouges
• Fibre de collagène : Jaunes

Hale

• Sialomucines et mucopolysaccharides acides : Bleu foncé
• Noyaux : Bleu
• Autres éléments tissulaires : Différentes teintes de mauve-violet

Fouchet

• Bile pigments emerald to blue-green
• Muscle yellow
• Collagen red

Verhoeff

• Elastic tissue fibers black
• Nuclei red