Fixateurs

Composantes: https://www.emsdiasum.com/

• 16220 Glutaraldehyde 25% Aqueous Solution
• 12300-25 Cacodylic Acid- Sodium Salt, TrihydrateSodium Cacodylate
• 15710 Formaldehyde Aqueous Solution 16%

1. Faire solution stock cacodylate 0.2M, pH 7.2-7.4
2. Ajouter :
   • 20 ml Cacodylate 0.2M (0.1M final)
   • 10 ml Formaldehyde 16% (4% final)
   • 4 ml Glutaraldehyde 25% (2.5% final)
   • 6 ml H20 (pour compléter à 40 ml)

Se conserve 6 mois à 4C.¹⁾

Liens: https://biotech.unl.edu/tem-fixation-protocols-microscopy