MICROSCOPIE

Plateforme d'imagerie et microscopie de l'IBIS

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en:protocoles:fixateurs

Fixatives

Fixative for electron microscopy

Routine Fixatives

Components: https://www.emsdiasum.com/

  • 16220 Glutaraldehyde 25% Aqueous Solution
  • 12300-25 Cacodylic Acid- Sodium Salt, TrihydrateSodium Cacodylate
  • 15710 Formaldehyde Aqueous Solution 16%
  1. Make 0.2M cacodylate stock solution, pH 7.2-7.4
  2. Add :
    • 20ml Cacodylate 0.2M (final 0.1M)
    • 10 ml Formaldehyde 16% (4% final)
    • 4 ml Glutaraldehyde 25% (2.5% final)
    • 6 ml H20 (to complete to 40 ml)
en/protocoles/fixateurs.txt · Last modified: 2022/05/24 13:29 by Administrateur

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